科学家建立可分澳门永利官网泌CSF的屏障类器官2020-06-15 16:04

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本期文章:《科学》:Online/在线发表

英国MRC的分子生物学实验室Madeline A. Lancaster研究团队取得一项新突破。他们建立人中枢神经系统中,可产生脑脊液(CSF)的屏障类器官。相关论文于2020年6月11日发表在《科学》杂志上。

他们建立了具有选择性屏障和自足隔室中CSF样液体分泌的人脉络丛(ChP)类器官。他们表明,这种体外屏障对小分子表现出与体内相同的选择性,澳门永利网站,并且ChP-CSF类器官可以预测新型化合物的CNS渗透性。ChP-CSF类器官的转录组学和蛋白质组学特征显示出与体内的高度相似性。

最后,澳门永利网站,单细胞转录组学和蛋白质组学分析的交叉发现了以前未知的特化上皮亚型产生的关键人类CSF成分。

据了解,CSF是一种至关重要的液体,澳门永利网址,可提供营养、信号分子,并清除大脑中的有毒副产物。脑脊液由ChP产生,ChP是一种保护性上皮屏障,也可防止血液自由进入。

附:英文原文

Title: Human CNS barrier-forming organoids with cerebrospinal fluid production

Author: Laura Pellegrini, Claudia Bonfio, Jessica Chadwick, Farida Begum, Mark Skehel, Madeline A. Lancaster

Issue&Volume: 2020/06/11

Abstract: Abstract Cerebrospinal fluid (CSF) is a vital liquid, providing nutrients, signaling molecules, and clearing out toxic byproducts from the brain. The CSF is produced by the choroid plexus (ChP), a protective epithelial barrier that also prevents free entry from the blood. Here, we establish human ChP organoids with a selective barrier and CSF-like fluid secretion in self-contained compartments. We show that this in vitro barrier exhibits the same selectivity to small molecules as in vivo, and that ChP-CSF organoids can predict CNS permeability of novel compounds. The transcriptomic and proteomic signature of ChP-CSF organoids reveal a high degree of similarity to in vivo. Finally, the intersection of single cell transcriptomics and proteomic analysis uncovers key human CSF components produced by previously unidentified specialized epithelial subtypes.

DOI: 10.1126/science.aaz5626

Source: https://science.sciencemag.org/content/early/2020/06/10/science.aaz5626

期刊信息

Science:《科学》,创刊于1880年。隶属于美国科学促进会,最新IF:41.037

官方网址:https://www.sciencemag.org/


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